John Antos, PhD

(360) 650-2271 | CB 440

View Profile

*WWU undergraduate co-authors, #WWU M.S. co-author

  • *Boyko, K.V.; #Rosenkranz, E.A.; *Smith, D.M.; #Miears, H.L.; #Oueld es cheikh, M.; *Lund, M.Z.; Young, J.C.; Reardon, P.N.; Okon, M.; Smirnov, S.L.; Antos, J.M. “Sortase-Mediated Segmental Labeling: A Method for Segmental Assignment of Intrinsically Disordered Regions in Proteins.” PLOS ONE 2021, 16, e0258531.
  • *Piper, I.M.; #Struyvenberg, S.A.; *Valgardson, J.D.; *Johnson, D.A.; *Gao, M.; *Johnston, K.; *Svendsen J.E.; *Kodama, H.M.; Hvorecny, K.L.; Antos, J.M.; Amacher, J.F. “Sequence Variation in the β7-β8 Loop of Bacterial Class A Sortase Enzymes Alters Substrate Selectivity.” J. Biol. Chem. 2021, 297, 100981.
  • #Reed, S.A.; *Brzovic, D.A.; *Takasaki, S.S.; *Boyko, K.V.; Antos, J.M. “Efficient Sortase-Mediated Ligation using a Common C-terminal Fusion Tag.” Bioconjugate Chem. 2020, 31, 1463-1473.
  • #Miears, H.L.; #Gruber, D.R.; #Horvath, N.M.; Antos, J.M.; Young, J.; #Sigurjonsson, J.P.; *Klem, M.L.; *Rosenkranz, E.A.; Okon, M.; McKnight, C.J.; Vugmeyster, L.; Smirnov, S.L. “Plant Villin Headpiece Domain Demonstrates a Novel Surface Charge Pattern and High Affinity for F-Actin.”  Biochemistry, 2018, 57, 1690-1701. 
  • #Nikghalb, K.D.; *Horvath, N.M.; *Prelesnik, J.L.; #Banks, O.G.B.; *Filipov, P.A.; *Row, R.D.; *Roark, T.J.; Antos, J.M. “Expanding the Scope of Sortase-Mediated Ligations Using Sortase Homologues.” ChemBioChem 2018, 19, 185-195.
  • Antos, J.M., Ingram, J., Fang, T., Pishesha, N., Truttmann, M.C., & Ploegh, H.L. "Site-specific protein labeling via sortase-mediated transpeptidation." Curr. Prot. Prot. Sci., 2017, 89, 15.3.1–15.3.19.
  • Antos, J.M.; Truttmann, M.C.; Ploegh, H.L. “Recent Advances in Sortase-Catalyzed Ligation Methodology.” Curr. Opin. Struct. Biol. 2016, 38, 111-118.
  • *Row, R.D.; *Roark, T.J.; *Philip, M.C.; *Perkins, L.L.; Antos, J.M. "Enhancing the Efficiency of Sortase–Mediated Ligations Through Nickel–Peptide Complex Formation." Chem. Commun., 2015, 51, 12548-12551. 
  • *Atterberry, P.N.; *Roark, T.J.; #Severt, S.Y.; *Schiller, M.L.; Antos, J.M.; Murphy, A.R. “Sustained Delivery of the Chemokine CXCL12 from Chemically Modified Silk Hydrogels.” Biomacromolecules, 2015, 16(5), 1582-1589.


Prior to WWU

  • Sinisi, A; Popp, M.W.; Antos J.M.; Pansegrau W.; Savino, S.; Nissum, M.; Rappuoli, R.; Ploegh H.L.; Buti L.; “Development of an influenza virus protein array using sortagging technology.” Bioconjugate Chem. 2012, 23(6), 1119-1126.
  • Guimaraes, C.P.; Carette, J.E.; Varadarajan, M.; Antos, J.M.; Popp, M.W.; Spooner, E.; Brummelkamp, T.R.; Ploegh, H.L.; “Identification of host cell factors required for intoxication through use of modified cholera toxin.” J. Cell Biol. 2011, 195(5), 751-764. 
  • Bolscher, J.G.; Oudhoff, M.J.; Nazmi, K.; Antos, J.M.; Guimaraes, C.P.; Spooner, E.; Haney, E.F.; Garcia-Vallejo, J.J.; Vogel, H.J.; Van't Hof W.; Ploegh, H.L.; Veerman, E.C.; “Sortase as a tool for high-yield histatin cyclization.” FASEB J., 2011, 25(8), 2650-2658.
  • Artavanis-Tsakonas, K.; Weihofen, W.; Antos, J.M.; Coleman, B.; Comeaux, C.; Duraisingh, M.; Gaudet, R.; Ploegh, H.L.  “Characterization and structural studies of the Plasmodium falciparum ubiquitin and Nedd8 hydrolase UCHL3.”  J. Biol. Chem., 2010, 285(9), 6857-66.
  • Antos, J.M.; Chew, G.L.; Guimaraes, C.; Yoder, N.C.; Grotenbreg, G.M.; Popp, M.W.; Ploegh, H.L.  “Site-specific N- and C-terminal labeling using sortases of different specificity.” J. Am. Chem. Soc., 2009, 131(31), 10800-10801.
  • Antos, J.M.; Popp, M.W.; Ernst, R.; Chew, G.L.; Spooner, E.; Ploegh, H.L.  “A straight path to circular proteins.” J. Biol. Chem., 2009 284(23), 16028-16036. 
  • Antos, J.M.; McFarland, J.M.; Iavarone, A.T.; Francis, M.B.  “Chemoselective tryptophan labeling with rhodium carbenoids at mild pH.”  J. Am. Chem. Soc. 2009, 131(17), 6301-6308.
  • Popp, M.W.; Antos, J.M.; Ploegh, H.L.  “Site-specific protein labeling via sortase-mediated transpeptidation.”  Curr. Prot. Pro. Sci. 2009, Chapter 15, Unit 15.3.
  • Antos, J.M.; Miller, G.M.; Grotenbreg, G.M.; Ploegh, H.L.  “Lipid modification of proteins through sortase catalyzed transpeptidation.”  J. Am. Chem. Soc.  2008, 130(48), 16338-16343.
  • Popp, M.W.; Antos, J.M.; Grotenbreg, G.M.; Spooner, E.; Ploegh, H.L.  “Sortagging:  A versatile method for protein labeling.”  Nat. Chem. Bio. 2007, 3(11), 707-708.
  • Antos, J.M.; Francis, M.B.  “Transition metal catalyzed methods for site-selective protein modification.”  Curr. Opin. Chem. Biol.  2006, 10(3), 253-262. 
  • Antos, J.M.; Francis, M.B.  “Selective tryptophan modification with rhodium carbenoids in aqueous solution.” J. Am. Chem. Soc. 2004, 126(33), 10256-10257.


  • Ploegh, H.L.; Antos, J.M.; Popp, M.W.; Guimaraes, C. “Methods for ligation and uses thereof.” United States Patent Application 13/145,644, filed February 1, 2010.